PROTOPLAST PRODUCTION IN CHONDRUS-CRISPUS GAMETOPHYTES (GIGARTINALES, RHODOPHYTA)

被引：50

作者：

LEGALL, Y ^{[1
]}

BRAUD, JP ^{[1
]}

KLOAREG, B ^{[1
]}

机构：

[1] SANOFI BIOIND,F-50500 CARENTAN,FRANCE

来源：

PLANT CELL REPORTS | 1990年 / 8卷 / 10期

关键词：

D O I：

10.1007/BF00270058

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplasts were isolated from female gametophytes of Chondrus crispus (Stackh.) using commercial cellulase and various carrageenases prepared from marine bacteria. Depending on the nature of the donor tissue (apices or whole thallus, wild or cultivated strains), yields ranged from 1.0-8.5×108 protoplasts per gram of fresh tissue. Preincubating the tissue with a potassium chelator, Kryptofix 222, enhanced protoplast yields by 30-50 %. Based on staining with fluorescein diacetate most protoplasts were viable. A few protoplasts regenerated a cell wall and divided. © 1990 Springer-Verlag.

引用

页码：582 / 585

页数：4

共 21 条

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