DETERMINATION OF ALBUTEROL CONCENTRATIONS IN HUMAN PLASMA USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：31

作者：

BLAND, RE

TANNER, RJN

CHERN, WH

LANG, JR

POWELL, JR

机构：

[1] GLAXO INC,PHARMACEUT DEV,DEPT CLIN PHARMACOKINET,5 MOORE DR,RES TRIANGLE PK,NC 27709

[2] GLAXO GRP RES LTD,DEPT BIOCHEM PHARMACOL,WARE SG12 0DJ,HERTS,ENGLAND

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 07期

关键词：

Albuterol; fluorescence detection; high-performance liquid chromatography; human plasma; pharmacokinetic studies; routine sample analysis; solid-phase extraction;

D O I：

10.1016/0731-7085(90)80085-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast, simple, and accurate method for determining albuterol concentrations in human plasma has been developed and validated for use in routine clinical analyses. This method involves a solid-phase extraction procedure using silica cartridges and normal-phase high-performance liquid chromatography with fluorescence detection. Regression analysis showed the method was linear over the standard curve range 1-16 ng ml-1. The percent recovery for albuterol and the internal standard, bamethane, at 5 ng ml-1 was found to be ≫90%. The newly developed method has been applied in the analysis of plasma samples from patients and healthy volunteers. © 1990.

引用

页码：591 / 596

页数：6

共 13 条

[1] AUTOMATED EXTRACTION OF DRUGS FROM BIOLOGICAL-FLUIDS [J].