ORGAN-CULTURE OF IXODID-TICK SALIVARY-GLANDS

被引：5
作者：
KAUFMAN, WR
机构：
[1] Department of Zoology, University of Alberta, Edmonton, T6G 2E9, Alberta
来源：
EXPERIMENTAL & APPLIED ACAROLOGY | 1990年 / 9卷 / 1-2期
关键词：
D O I：
10.1007/BF01198985
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
This work describes an organ-culture method for isolated salivary glands of the ixodid tick Amblyomma hebraeum Koch. The support medium is a TC 199-1% agarose gel with no hormonal or undefined supplements. I used a fluid-transport assay for measuring viability of salivary glands. When cultured at 26°C, female glands secreted fluid at 65% on day 2,59% on day 4 and 42% on day 6 post-culture compared to glands tested on day 0. Fluid secretory rates in culture were higher than rates of glands from partially fed ticks (about 180-250 mg) on days 2, 4 or 6 post-removal from the host. Salivary glands from fed males cultured for 4 days lost only 5% of their fluid secretory competence, whereas glands dissected from males 4 days post-removal lost 63% of their fluid secretory competence. © 1990 Elsevier Science Publishers B.V.
引用
收藏
页码：79 / 86
页数：8
相关论文
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