ORGAN-CULTURE OF IXODID-TICK SALIVARY-GLANDS

被引：5
作者：
KAUFMAN, WR
机构：
[1] Department of Zoology, University of Alberta, Edmonton, T6G 2E9, Alberta
来源：
EXPERIMENTAL & APPLIED ACAROLOGY | 1990年 / 9卷 / 1-2期
关键词：
D O I：
10.1007/BF01198985
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
This work describes an organ-culture method for isolated salivary glands of the ixodid tick Amblyomma hebraeum Koch. The support medium is a TC 199-1% agarose gel with no hormonal or undefined supplements. I used a fluid-transport assay for measuring viability of salivary glands. When cultured at 26°C, female glands secreted fluid at 65% on day 2,59% on day 4 and 42% on day 6 post-culture compared to glands tested on day 0. Fluid secretory rates in culture were higher than rates of glands from partially fed ticks (about 180-250 mg) on days 2, 4 or 6 post-removal from the host. Salivary glands from fed males cultured for 4 days lost only 5% of their fluid secretory competence, whereas glands dissected from males 4 days post-removal lost 63% of their fluid secretory competence. © 1990 Elsevier Science Publishers B.V.
引用
收藏
页码：79 / 86
页数：8
相关论文
共 18 条
  • [1] Bell L.J., Organ culture of Rhipicephalus appendiculatus with maturation of Theileria parva in tick salivary glands in vitro, Acta Trop., 37, pp. 319-325, (1980)
  • [2] Brown S.J., Askenase P.W., Characterization of Amblyomma americanum-derived salivary gland proteins responsible for the elicitation of host immunity, Morphology, Physiology and Behavioural Biology of Ticks, pp. 300-328, (1986)
  • [3] Fawcett D.W., Doxsey S., Buscher G., Salivary gland of the tick vector (R. appendiculatus) of East Coast Fever. II. Cellular basis for fluid secretion in the type III acinus, Tissue Cell, 13, pp. 231-253, (1981)
  • [4] Fawcett D.W., Buscher G., Doxsey S., Salivary gland of the tick vector (R. appendiculatus ) of East Coast Fever. III. The ultrastructure of sporogony in Theileria parva, Tissue Cell, 14, pp. 183-206, (1982)
  • [5] Feldman-Muhsam B., Borut S., Saliternike-Givant S., Salivary secretion of the male tick during copulation, J. Insect Physiol., 16, pp. 1945-1949, (1970)
  • [6] Harris R.A., Kaufman W.R., Hormonal control of salivary gland degeneration in the ixodid tick Amblyomma hebraeum, J. Insect Physiol., 27, pp. 241-248, (1981)
  • [7] Harris R.A., Kaufman W.R., Neural involvement in the control of salivary gland degeneration in the ixodid tick Amblyomma hebraeum, J. Exp. Biol., 109, pp. 281-290, (1984)
  • [8] Harris R.A., Kaufman W.R., Ecdysteroids: possible candidates for the hormone which triggers salivary gland degeneration in the ixodid tick Amblyomma hebraeum, Experientia, 41, pp. 740-742, (1985)
  • [9] Hoffmann G., Haltung babesieninfizierter Zeckengewebe in künstlichern Nährmedium, Z. Angew. Entomol., 71, pp. 26-34, (1972)
  • [10] Hoffmann G., Schein E., Jagow M., Untersuchungen an extirpierten und in der Kultur gehaltenen Zeckengeweben, Tropenmed. Parasitol., 21, pp. 46-61, (1970)
12