Detection of bla CTX-M, bla TEM-01 and bla SHV Genes in Multidrug Resistant Uropathogenic E. coli Isolated from Patients with Recurrent Urinary Tract Infections

Introduction: Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae that give antibacterial resistance to 3rd generations cephalosporin and broad-spectrum penicillin, using ESBL genes CTX-M, TEM, SHV, which are encoded in bacterial plasmid genome. This study was concerned with detection of bla CTX-M, bla TEM-01 and bla SHV genes in multidrug resistant uropathogenic E. coli isolates in adult patients with recurrent Urinary Tract Infections (UTIs). Material and Methods: Total 47 of uropathogenic E. coli (UPEC) was isolated from patients with urinary tract infection who attended Azadi Teaching Hospital in Duhok city. The isolates were collected from June 2016 to December 2016 and investigated by using antimicrobial susceptibility assay according to Clinical and Laboratory Standards Institute (CLSI) guidelines and by using Phoenix (BD). All these UPEC isolates were investigated for subgroups gene of bla CTX-M such as CTX-M-14, 15, 24, 27, bla SHV subgroups gene such as SHV-11, 12 and bla TEM-1 by using uniplex and multiplex PCR. All UPEC samples were screened by Double Disc Approximation (Synergy) test (DDAT) for detection of the positivity to ESBLs production. Results: Out of 47 UPEC isolates, bla CTX-M15 subgroup gene was detected in 72.3%, CTX-M14 was detected in 42.6%, bla subgroup gene CTX-M24 was detected in 27.7% and the last subgroup gene bla CTX-M27 was detected in 12.8%. Conclusion: The current study found that an acute uncomplicated UTI affects a large proportion of the population. The study confirmed that the E. coli to be a major uropathogen. ESBL encoding genes, the resistance among males were higher than females particularly the distribution of bla TEM genes.