DETERMINATION OF OFLOXACIN IN HUMAN PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORESCENCE DETECTION

被引：29

作者：

MACEK, J

PTACEK, P

机构：

[1] Pharmakl s.r.o., CZ-16902 Prague 6, KlinLab Building

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 673卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00263-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the determination of ofloxacin in human plasma was developed. Plasma proteins were precipitated with acetonitrile, the supernatant concentrated and injected into a reversed-phase C-18 column. Enoxacin was used as an internal standard. The fluorimetric detection was performed at 282 nm for excitation and 450 nm for emission. Limit of quantitation was 20 ng/ml and the calibration curve was linear up to 6900 ng/ml.

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页码：316 / 319

页数：4

相关论文

共 8 条

[1] A REPRODUCIBLE, SIMPLE AND SENSITIVE HPLC ASSAY FOR DETERMINATION OF OFLOXACIN IN PLASMA AND LUNG-TISSUE - APPLICATION IN PHARMACOKINETIC STUDIES [J].

FABRE, D ;

BRESSOLLE, F ;

KINOWSKI, JM ;

BOUVET, O ;

PAGANIN, F ;

GALTIER, M .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1994, 12 (11) :1463-1469

[2]

FOSS DL, 1994, J PHARM BIOMED ANAL, V12, P1325

[3] DETERMINATION OF OFLOXACIN IN PLASMA AND URINE BY LIQUID-CHROMATOGRAPHY [J].

LECOGUIC, A ;

BIDAULT, R ;

FARINOTTI, R ;

DAUPHIN, A .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 434 (01) :320-323

[4] DETERMINATION OF OFLOXACIN IN BRONCHOALVEOLAR LAVAGE FLUID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORIMETRIC DETECTION [J].

MATSUBAYASHI, K ;

UNE, T ;

OSADA, Y .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 495 :354-357

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF OFLOXACIN IN PLASMA AND URINE [J].

MIGNOT, A ;

LEFEBVRE, MA ;

FOURTILLAN, JB .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 430 (01) :192-197

[6] METHOD FOR THE DETERMINATION OF OFLOXACIN, A QUINOLONE CARBOXYLIC-ACID ANTIMICROBIAL, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

NOTARIANNI, LJ ;

JONES, RW .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 431 (02) :461-464

[7] DETERMINATION OF OFLOXACIN IN HUMAN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN SWITCHING [J].

OHKUBO, T ;

KUDO, M ;

SUGAWARA, K .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 573 (02) :289-293

[8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF (S)-(-)-OFLOXACIN AND ITS METABOLITES IN SERUM AND URINE USING A SOLID-PHASE CLEANUP [J].

OKAZAKI, O ;

AOKI, H ;

HAKUSUI, H .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1991, 563 (02) :313-322