PURIFICATION OF THE LYSR FAMILY REGULATOR, CLCR, AND ITS INTERACTION WITH THE PSEUDOMONAS-PUTIDA CLCABD CHLOROCATECHOL OPERON PROMOTER

被引：36

作者：

COCO, WM ^{[1
]}

PARSEK, MR ^{[1
]}

CHAKRABARTY, AM ^{[1
]}

机构：

[1] UNIV ILLINOIS, COLL MED, DEPT MICROBIOL & IMMUNOL, CHICAGO, IL 60612 USA

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 17期

关键词：

D O I：

10.1128/JB.176.17.5530-5533.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous studies have shown that the clcABD operon is under the transcriptional control of the LysR-type activator ClcR In this study, the conditions leading to its aggregation were avoided and ClcR was purified and confirmed by amino-terminal sequencing. Gel filtration indicated that ClcR exists as a dimer in solution. The DNase I footprint of ClcR was determined. The binding properties of ClcR and the catechol operon regulator, CatR, were compared.

引用

页码：5530 / 5533

页数：4

共 21 条

[1] BELASCO J, 1993, 93RD GEN M AM SOC MI
[2] CONTROL OF RNASE-E-MEDIATED RNA DEGRADATION BY 5'-TERMINAL BASE-PAIRING IN ESCHERICHIA-COLI
BOUVET, P
BELASCO, JG
[J]. NATURE, 1992, 360 (6403) : 488 - 491
[3] NUCLEOTIDE-SEQUENCE AND INITIAL FUNCTIONAL-CHARACTERIZATION OF THE CLCR GENE ENCODING A LYSR FAMILY ACTIVATOR OF THE CLCABD CHLOROCATECHOL OPERON IN PSEUDOMONAS-PUTIDA
COCO, WM
ROTHMEL, RK
HENIKOFF, S
CHAKRABARTY, AM
[J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (02) : 417 - 427
[4] COCO WM, 1994, THESIS U ILLINOIS CH
[5] ENGLARD S, 1990, METHOD ENZYMOL, V182, P285
[6] CONSERVED MOTIFS IN A DIVERGENT NOD BOX OF AZORHIZOBIUM-CAULINODANS ORS571 REVEAL A COMMON STRUCTURE IN PROMOTERS REGULATED BY LYSR-TYPE PROTEINS
GOETHALS, K
VANMONTAGU, M
HOLSTERS, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) : 1646 - 1650
[7] IMPROVED PREDICTIONS OF SECONDARY STRUCTURES FOR RNA
JAEGER, JA
TURNER, DH
ZUKER, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) : 7706 - 7710
[8] JAEGER JA, 1990, METHODS ENZYMOL, V183, P281
[9] CHARACTERIZATION OF ACINETOBACTER-CALCOACETICUS-CATM, A REPRESSOR GENE HOMOLOGOUS IN SEQUENCE TO TRANSCRIPTIONAL ACTIVATOR GENES
NEIDLE, EL
HARTNETT, C
ORNSTON, LN
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (10) : 5410 - 5421
[10] ROLES OF CATR AND CIS,CIS-MUCONATE IN ACTIVATION OF THE CATBC OPERON, WHICH IS INVOLVED IN BENZOATE DEGRADATION IN PSEUDOMONAS-PUTIDA
PARSEK, MR
SHINABARGER, DL
ROTHMEL, RK
CHAKRABARTY, AM
[J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (23) : 7798 - 7806

← 1 2 3 →