PROTEIN-SYNTHESIS IS REQUIRED FOR THE GENERATION OF OSCILLATORY CURRENT RESPONSES IN XENOPUS OOCYTE

In Xenopus oocyte, direct activation of G-proteins by AIF(4)(-) or injection of inositol 1,4,5-trisphosphate evoked Ca2+-gated Cl- current responses. The current responses were smooth and not oscillatory when tested immediately after the oocyte was isolated. After hours of incubation, the response became oscillatory. This change was prevented by cycloheximide, a protein synthesis inhibitor. Results indicate that the characteristics of Ca2+ signaling system have changed from not oscillatory to oscillatory with time and protein synthesis was required for this change. (C) 1994 Academic Press, Inc.