CHROMATOGRAPHIC BEHAVIOR OF THE CLOSO-[B12H12]2-DERIVATIVES ON HYDROXYETHYLMETHACRYLATE GELS

Halo derivatives (Cl, Br, I) of the closo-dodecahydro-dodecaborate anion, [B12H12]2-, and their positional isomers were separated on Separon HEMA hydroxyethylmetacrylate sorbents. Different effects on the separation process, including cations and anions in the mobile phase, the electrolyte concentration in the eluent, pH, addition of organic modifier and separation temperature, were studied. Optimum conditions for the separation were accomplished with Separon HEMA BIO 300 and 1000 sorbents using 0.1-0.5 M sodium perchlorate solutions in 0.01 M phosphate buffer (pH 8.5) as the mobile phase. The separated compounds were detected using direct UV detection in the range 200-210 nm.