THE COMPLETE AMINO-ACID-SEQUENCE OF THE HEMORRHAGIC FACTOR LHFII, A METALLOPROTEINASE ISOLATED FROM THE VENOM OF THE BUSHMASTER SNAKE (LACHESIS-MUTA-MUTA)

被引：58
作者：
SANCHEZ, EF
DINIZ, CR
RICHARDSON, M
机构：
[1] UNIV DURHAM,DEPT BIOL SCI,SCI LABS,S RD,DURHAM DH1 3LE,ENGLAND
[2] FDN EZEQUIEL DIAS,CTR PESQUISA & DESENVOLVIMENTO,BR-30550 BELO HORIZONTE,MG,BRAZIL
来源：
FEBS LETTERS | 1991年 / 282卷 / 01期
关键词：
SNAKE VENOM; AMINO ACID SEQUENCE; METALLOPROTEINASE; HEMORRHAGIC FACTOR; LACHESIS-MUTA-MUTA;
D O I：
10.1016/0014-5793(91)80472-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complete amino acid sequence the haemorrhagic agent LHFII, a Zn and Ca containing metalloproteinase isolated from the venom of the Bushmaster snake Lachesis muta muta was determined by automated and DABITC/PITC microsequencing of the intact protein, fragments derived by cleavage with cyanogen bromide, and peptides resulting from enzymatic digestions with trypsin and the protease from S. aureus V8. The protein is composed of 200 residues and exhibits considerable sequence homology with the haemorrhagic toxins from a number of other snake venoms, and some metalloproteinases in the region of the putative Zn-binding sites.
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页码：178 / 182
页数：5
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