CLONING OF DOUBLE STRANDED DNA CODING FOR HORDEIN POLYPEPTIDES

被引：10
作者：
BRANDT, A
机构：
[1] Department of Psysiology, Carlsberg Laboratory, Copenhagen Valby, DK-2500
来源：
CARLSBERG RESEARCH COMMUNICATIONS | 1979年 / 44卷 / 04期
关键词：
Hordein; messenger RNA; molecular cloning; reverse transcription;
D O I：
10.1007/BF02906159
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Double stranded cDNA was synthesized from a partially purified hordein mRNA preparation isolated from developing barley endosperm. The double stranded cDNA was inserted by homopolymeric tailing into the Hind III site of plasmid pBR 322. Following bacterial transformation one recombinant plasmid which contains an 850 base pair insert was shown to hybridize to the mRNAs coding for two of the B-hordein polypeptides. © 1979 Carlsberg Laboratory.
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页码：255 / 267
页数：13
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