QUANTITATION OF SUPEROXIDE PRODUCTION IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES FROM NORMALS AND 3 TYPES OF CHRONIC GRANULOMATOUS-DISEASE

被引：40

作者：

BORREGAARD, N ^{[1
]}

JOHANSEN, KS ^{[1
]}

ESMANN, V ^{[1
]}

机构：

[1] STATENS SERUM INST,DEPT CLIN MICROBIOL,DK-2300 COPENHAGEN S,DENMARK

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91612-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions for quantitating the superoxide producing capacity of polymorphonuclear leukocytes are given. It is shown that the production is partly dependent on glucose but independent on Mg2+, Ca2+ and extracellular pH in the range 6.6-8.2. The capacity of normal cells to produce superoxide is estimated to 13.4±0.13 SEM (n=9) femtomoles per min per cell, whereas superoxide production is absent in all cases of chronic granulomatous disease, irrespective of the type. It is suggested that at least 3 enzymes cooperate intimately in the superoxide producing system of the cell. This supports the theory of an electron transport chain of significance for oxygen consumption. © 1979.

引用

页码：214 / 219

页数：6

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