AGAR WELL DIFFUSION ASSAY TESTING OF BACTERIAL SUSCEPTIBILITY TO VARIOUS ANTIMICROBIALS IN CONCENTRATIONS NONTOXIC FOR HUMAN-CELLS IN CULTURE

被引：212

作者：

HOLDER, IA

BOYCE, ST

机构：

[1] Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH

[2] Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, OH

[3] Shriners Burns Institute, Cincinnati, OH

来源：

BURNS | 1994年 / 20卷 / 05期

关键词：

D O I：

10.1016/0305-4179(94)90035-3

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Previously, we showed that microbial susceptibility to antimicrobials in concentrations non-toxic for human cells in culture could be tested using the wet disc topical antimicrobial assay. In this report, wet disc assay and agar well diffusion assay results were compared testing the susceptibility of Ps. aeruginosa isolates from burn patients to concentrations of Polymyxin B non-toxic for cultured cells. Both assays were performed on the same agar plates. No differences in results were observed. Further agar well diffusion assay testing showed that susceptibility/resistance could be demonstrated when testing several antimicrobials in concentrations non-toxic for cultured cells against a variety of bacteria isolated from burn patients. Therefore, the more familiar agar well diffusion as well as the wet disc assay can be used to test microbial susceptibility to these concentrations of antimicrobials.

引用

页码：426 / 429

页数：4

共 18 条

[1]

Carver, Leigh, Keratinocyte grafts and skin equivalents, Int J Dermatol, 30, pp. 540-541, (1991)

[2]

Gallico, O'Conner, Compton, Et al., Permanent coverage of large burn wounds with autologous cultured human epithelium, New Engl J Med, 311, pp. 448-451, (1984)

[3]

Hansbrough, Boyce, Cooper, Et al., Burn wound closure with cultured human keratinocytes and fibroblasts attached to a collagen-GAG substrate, JAMA, 262, pp. 2125-2130, (1989)

[4]

Hull, Finley, Miller, Coverage of burns with bi-layered skin equivalents: a preliminary clinical trial, Surgery, 107, pp. 496-502, (1990)

[5]

Cooper, Boyce, Hansbrough, Et al., Cytotoxicity to cultured human keratinocytes (HK) of topical antimicrobial agents, J Surg Res, 48, pp. 190-195, (1990)

[6]

Lineweaver, McMorris, Soucy, Et al., Cellular and bacterial toxicities of topical antimicrobials, Plastic and Reconstructive Surgery, 75, pp. 394-396, (1985)

[7]

Barillo, Nangle, Farrel, Preliminary experience with cultured epidermal autograft in a community hospital burn unit, J Burn Care Rehabil, 13, pp. 158-165, (1992)

[8]

Boyce, Holder, Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity, Plast Reconstr Surg, 92, pp. 493-500, (1993)

[9]

Boyce, Holder, Cytotoxicity testing of human keratinocytes and fibroblasts to topical antimicrobial agents for use with cultured skin grafts, Proc Am Burn Assoc, (1993)

[10]

Holder, The wet disc antimicrobial solution assay: an in vitro method to test efficacy of antimicrobial solutions for topical use, J Burn Care Rehabil, 10, pp. 203-208, (1989)

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