EXTENSION OF DNA TRIPLE-HELIX FORMATION TO A NEIGHBORING (AT)(N) SITE

被引：12

作者：

CHANDLER, SP ^{[1
]}

FOX, KR ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND

来源：

FEBS LETTERS | 1995年 / 360卷 / 01期

基金：

英国医学研究理事会;

关键词：

DNA TRIPLE HELIX; G-CENTER-DOT-TA TRIPLET; DNA SEQUENCE RECOGNITION;

D O I：

10.1016/0014-5793(95)00069-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A(11)(AT)(6) .(AT)(6)T-11, using oligonucleotides designed to form parallel T . AT and G . TA triplets, We find that, although (TG), does not form a complex with (AT)(6) .(AT)(6), T-11(TG)(6) forms a stable structure producing a clear footprint which includes the (AT)(6) portion of the target site, This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a tripler-binding ligand.,

引用

页码：21 / 25

页数：5

共 22 条

[21] DNA TRIPLE-HELIX SPECIFIC INTERCALATORS AS ANTIGENE ENHANCERS - UNFUSED AROMATIC CATIONS
WILSON, WD
TANIOUS, FA
MIZAN, S
YAO, SJ
KISELYOV, AS
ZON, G
STREKOWSKI, L
[J]. BIOCHEMISTRY, 1993, 32 (40) : 10614 - 10621
[22] ELUCIDATION OF THE SEQUENCE-SPECIFIC 3RD-STRAND RECOGNITION OF 4 WATSON-CRICK BASE-PAIRS IN A PYRIMIDINE TRIPLE-HELIX MOTIF - T-BULLET-AT, C-BULLET-GC, T-BULLET-CG, AND G-BULLET-TA
YOON, K
HOBBS, CA
KOCH, J
SARDARO, M
KUTNY, R
WEIS, AL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) : 3840 - 3844

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