EXTENSION OF DNA TRIPLE-HELIX FORMATION TO A NEIGHBORING (AT)(N) SITE

被引：12

作者：

CHANDLER, SP ^{[1
]}

FOX, KR ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND

来源：

FEBS LETTERS | 1995年 / 360卷 / 01期

基金：

英国医学研究理事会;

关键词：

DNA TRIPLE HELIX; G-CENTER-DOT-TA TRIPLET; DNA SEQUENCE RECOGNITION;

D O I：

10.1016/0014-5793(95)00069-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A(11)(AT)(6) .(AT)(6)T-11, using oligonucleotides designed to form parallel T . AT and G . TA triplets, We find that, although (TG), does not form a complex with (AT)(6) .(AT)(6), T-11(TG)(6) forms a stable structure producing a clear footprint which includes the (AT)(6) portion of the target site, This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a tripler-binding ligand.,

引用

页码：21 / 25

页数：5

共 22 条

[1] 2ND STRUCTURAL MOTIF FOR RECOGNITION OF DNA BY OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION [J].

BEAL, PA ;

DERVAN, PB .

SCIENCE, 1991, 251 (4999) :1360-1363

[2]

CASSIDY SA, 1994, IN PRESS BIOCHEMISTR

[3] TRIPLE-HELIX FORMATION AT A8XA8.T8YT8 [J].

CHANDLER, SP ;

FOX, KR .

FEBS LETTERS, 1993, 332 (1-2) :189-192

[4] HUMAN THERAPEUTICS BASED ON TRIPLE HELIX TECHNOLOGY [J].

CHUBB, JM ;

HOGAN, ME .

TRENDS IN BIOTECHNOLOGY, 1992, 10 (04) :132-136

[5] WRAPPING OF GENOMIC POLYDA.POLYDT TRACTS AROUND NUCLEOSOME CORE PARTICLES [J].

FOX, KR .

NUCLEIC ACIDS RESEARCH, 1992, 20 (06) :1235-1242

[6] INTERACTION OF MITHRAMYCIN WITH DNA FRAGMENTS COMPLEXED WITH NUCLEOSOME CORE PARTICLES - COMPARISON WITH DISTAMYCIN AND ECHINOMYCIN [J].

FOX, KR ;

CONS, BMG .

BIOCHEMISTRY, 1993, 32 (28) :7162-7171

[7] RECOGNITION OF THYMINE.ADENINE BASE-PAIRS BY GUANINE IN A PYRIMIDINE TRIPLE HELIX MOTIF [J].

GRIFFIN, LC ;

DERVAN, PB .

SCIENCE, 1989, 245 (4921) :967-971

[8] FLANKING SEQUENCE EFFECTS WITHIN THE PYRIMIDINE TRIPLE-HELIX MOTIF CHARACTERIZED BY AFFINITY CLEAVING [J].

KIESSLING, LL ;

GRIFFIN, LC ;

DERVAN, PB .

BIOCHEMISTRY, 1992, 31 (10) :2829-2834

[9] CATION AND SEQUENCE EFFECTS ON STABILITY OF INTERMOLECULAR PYRIMIDINE-PURINE-PURINE TRIPLEX [J].

MALKOV, VA ;

VOLOSHIN, ON ;

SOYFER, VN ;

FRANKKAMENETSKII, MD .

NUCLEIC ACIDS RESEARCH, 1993, 21 (03) :585-591

[10] TRIPLEX DNA FINALLY COMES OF AGE [J].

MOFFAT, AS .

SCIENCE, 1991, 252 (5011) :1374-1375

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