CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN CDNA FOR THE ALPHA(1C)-ADRENERGIC RECEPTOR

被引：167
|
作者：
HIRASAWA, A
HORIE, K
TANAKA, T
TAKAGAKI, K
MURAI, M
YANO, J
TSUJIMOTO, G
机构：
[1] NATL CHILDRENS MED RES CTR,DEPT MOLEC CELL PHARMACOL,3-35-31 TAISHIDO,SETAGAYA KU,TOKYO 154,JAPAN
[2] NIPPON SHINYAKU CO LTD,DEPT MOLEC BIOL,RES LABS,MINAMI KU,KYOTO 601,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 02期
关键词：
D O I：
10.1006/bbrc.1993.2130
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned human α1C-adrenergic receptor from human prostate cDNA library. The deduced amino acid sequence of the clone (P2C4) encodes a protein of 466 amino acids that showed strong sequence homology to the previously cloned bovine α1C-adrenergic receptor. The radioligand binding properties of P2C4 expressed in COS-7 cells were very similar to those of bovine α1C-adrenergic receptor. With reverse-transcription polymerase chain reaction assay, we observed α1C-adrenergic receptor transcripts in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. The data show that the clone P2C4 encodes a human α1C-adrenergic receptor cDNA, and the receptor subtype is expressed not widely but localized in several human tissues. © 1993 Academic Press, Inc.
引用
收藏
页码：902 / 909
页数：8
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