CRYSTALLIZATION OF THREONYL-TRANSFER-RNA SYNTHETASE FROM THERMUS-THERMOPHILUS AND PRELIMINARY CRYSTALLOGRAPHIC DATA

被引：6
作者：
CURA, V
KERN, D
MITSCHLER, A
MORAS, D
机构：
[1] UNIV STRASBOURG 1, IGBMC, CNRS, UPR 9004, INSERM, F-67404 ILLKIRCH GRAFFENSTADEN, FRANCE
[2] SMBMR, CNRS, UPR 9002, IBMC, F-67084 STRASBOURG, FRANCE
来源：
FEBS LETTERS | 1995年 / 374卷 / 01期
关键词：
AMINOACYL-TRANSFER-RNA SYNTHETASE; THERMUS THERMOPHILUS; CRYSTALLIZATION; TRANSFER-RNA AMINOACYLATION;
D O I：
10.1016/0014-5793(95)01089-W
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Threonyl-tRNA synthetase from Thermus thermophilus (ttTRS) has been overproduced in Escherichia coli, purified and crystallized in solutions containing ammonium sulfate and glycerol. The crystals grew in the orthorhombic space group C222(1) with unit cell dimensions a = 119.5 Angstrom, b = 120.0 Angstrom, c = 317.5 Angstrom. The asymmetric unit is constituted of two monomers and the crystals contain 66% solvent. This paper reports the first crystals of ttTRS and preliminary crystallographic results since the presumed crystals of ttTRS described in a previous paper [1] were crystals of aspartyl-tRNA synthetase [2].
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收藏
页码：110 / 112
页数：3
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