REVERSED-PHASE PLANAR CHROMATOGRAPHY OF DANSYL DL AMINO-ACIDS WITH BOVINE SERUM-ALBUMIN IN THE MOBILE PHASE

The chromatographic behavior of twelve dansyl DL amino acids, one D isomer and eleven L isomers on RP18W/UV254, RP18W/F254s, and Sil C18-50 UV254 plates developed with aqueous - organic solutions containing bovine serum albumin (BSA) as chiral complexing agent has been extensively investigated. Enantiomeric resolution is highly dependent on mobile phase pH and ionic strength, and on the concentration of both BSA and organic modifier. Ali the racemates have been resolved within a development time of 1 h 30 min. The selectivity factors (alpha) for the dansyl amino acids have been compared with those from planar chromatography for the corresponding DNP, DNPy, and Fmoc amino acids, and with those of the same dansyl derivatives on a column prepared from BSA bound to silica gel.