REVERSED-PHASE PLANAR CHROMATOGRAPHY OF DANSYL DL AMINO-ACIDS WITH BOVINE SERUM-ALBUMIN IN THE MOBILE PHASE

被引:30
|
作者
LEPRI, L
COAS, V
DESIDERI, PG
SANTIANNI, D
机构
[1] Department of Public Health, Epidemiology, and Environmental Analytical Chemistry, University of Florence, Florence, 50121
关键词
REVERSED PHASE TLC; BOVINE SERUM ALBUMIN; DANSYL DL; D-AMINO; AND L-AMINO ACIDS;
D O I
10.1007/BF02263881
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The chromatographic behavior of twelve dansyl DL amino acids, one D isomer and eleven L isomers on RP18W/UV254, RP18W/F254s, and Sil C18-50 UV254 plates developed with aqueous - organic solutions containing bovine serum albumin (BSA) as chiral complexing agent has been extensively investigated. Enantiomeric resolution is highly dependent on mobile phase pH and ionic strength, and on the concentration of both BSA and organic modifier. Ali the racemates have been resolved within a development time of 1 h 30 min. The selectivity factors (alpha) for the dansyl amino acids have been compared with those from planar chromatography for the corresponding DNP, DNPy, and Fmoc amino acids, and with those of the same dansyl derivatives on a column prepared from BSA bound to silica gel.
引用
收藏
页码:297 / 301
页数:5
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