ISOTACHOPHORESIS OF PROTEINS IN UNCOATED OPEN-TUBULAR FUSED-SILICA CAPILLARIES WITH A SIMPLE APPROACH FOR COLUMN CONDITIONING

被引：15

作者：

GEBAUER, P

THORMANN, W

机构：

[1] UNIV BERN, DEPT CLIN PHARMACOL, MURTENSTR 35, CH-3010 BERN, SWITZERLAND

[2] CZECHOSLOVAK ACAD SCI, INST ANALYT CHEM, CS-61142 BRNO, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 558卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)80009-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The isotachophoretic determination of proteins in uncoated open-tubular fused-silica capillaries of 50 and 75-mu-m I.C. with on-column multi-wavelength detection is reported. Small amounts of hydroxypro-pylmethylcellulose added to the leader provide an efficient method of dynamic column conditioning which allows the high-resolution isotachophoretic determination of most proteins to be performed in the presence of an electro-osmotic flow. Different approaches for cationic and anionic analyses are discussed and illustrated with selected examples.

引用

收藏

页码：423 / 429

页数：7

相关论文

共 15 条

[1] ISOTACHOPHORESIS WITH ELECTROOSMOTIC FLOW - OPEN VERSUS CLOSED SYSTEMS [J].

BECKERS, JL ;

EVERAERTS, FM ;

ACKERMANS, MT .

JOURNAL OF CHROMATOGRAPHY, 1991, 537 (1-2) :429-442

[2] PURIFICATION OF OVALBUMIN AND LYSOZYME FROM A COMMERCIAL PRODUCT BY RECYCLING ISOTACHOPHORESIS [J].

CASLAVSKA, J ;

GEBAUER, P ;

THORMANN, W .

JOURNAL OF CHROMATOGRAPHY, 1991, 585 (01) :145-152

[3]

Deml M., 1988, ANAL ISOTACHOPHORESI

[4]

EVERAERTS FM, 1976, J CHROMATOGR LIBRARY, V6

[5] ISOTACHOPHORESIS IN OPEN-TUBULAR FUSED-SILICA CAPILLARIES WITH ON-COLUMN MULTIWAVELENGTH DETECTION [J].

GEBAUER, P ;

THORMANN, W .

JOURNAL OF CHROMATOGRAPHY, 1991, 545 (02) :299-305

[6] CARRIER-FREE ZONE ELECTROPHORESIS, DISPLACEMENT ELECTROPHORESIS AND ISOELECTRIC-FOCUSING IN A HIGH-PERFORMANCE ELECTROPHORESIS APPARATUS [J].

HJERTEN, S ;

ELENBRING, K ;

KILAR, F ;

LIAO, JL ;

CHEN, AJC ;

SIEBERT, CJ ;

ZHU, MD .

JOURNAL OF CHROMATOGRAPHY, 1987, 403 :47-61

[7] HIGH-PERFORMANCE DISPLACEMENT ELECTROPHORESIS IN 0.025-MM TO 0.050-MM CAPILLARIES COATED WITH A POLYMER TO SUPPRESS ADSORPTION AND ELECTROENDOSMOSIS [J].

HJERTEN, S ;

KIESSLINGJOHANSSON, M .

JOURNAL OF CHROMATOGRAPHY, 1991, 550 (1-2) :811-822

[8] EFFECT OF ELECTROOSMOSIS ON DETECTION IN ISOTACHOPHORESIS [J].

REIJENGA, JC ;

ABEN, GVA ;

VERHEGGEN, TPEM ;

EVERAERTS, FM .

JOURNAL OF CHROMATOGRAPHY, 1983, 260 (02) :241-254

[9] CAPILLARY ISOTACHOPHORESIS WITH UV AND TANDEM MASS-SPECTROMETRIC DETECTION FOR PEPTIDES AND PROTEINS [J].

SMITH, RD ;

FIELDS, SM ;

LOO, JA ;

BARINAGA, CJ ;

UDSETH, HR ;

EDMONTS, CG .

ELECTROPHORESIS, 1990, 11 (09) :709-717

[10] CAPILLARY ZONE ELECTROPHORESIS AND ISOTACHOPHORESIS MASS-SPECTROMETRY OF POLYPEPTIDES AND PROTEINS BASED UPON AN ELECTROSPRAY IONIZATION INTERFACE [J].

SMITH, RD ;

LOO, JA ;

BARINAGA, CJ ;

EDMONDS, CG ;

UDSETH, HR .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :211-232