PERMETHYLATION AND TANDEM MASS-SPECTROMETRY OF OLIGOSACCHARIDES HAVING FREE HEXOSAMINE - ANALYSIS OF THE GLYCOINOSITOL PHOSPHOLIPID ANCHOR GLYCAN FROM THE SCRAPIE PRION PROTEIN

被引：57
作者：
BALDWIN, MA
STAHL, N
REINDERS, LG
GIBSON, BW
PRUSINER, SB
BURLINGAME, AL
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143
[3] UNIV CALIF SAN FRANCISCO,CTR LIVER,SAN FRANCISCO,CA 94143
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 01期
关键词：
D O I：
10.1016/0003-2697(90)90405-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPSc GPI glycan. © 1990.
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页码：174 / 182
页数：9
相关论文
共 35 条
[1]   SECONDARY ION MASS-SPECTROMETRY WITH CESIUM ION PRIMARY BEAM AND LIQUID TARGET MATRIX FOR ANALYSIS OF BIOORGANIC COMPOUNDS [J].
ABERTH, W ;
STRAUB, KM ;
BURLINGAME, AL .
ANALYTICAL CHEMISTRY, 1982, 54 (12) :2029-2034
[2]   FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY [J].
BARBER, M ;
BORDOLI, RS ;
SEDGWICK, RD ;
TYLER, AN .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) :325-327
[3]   SCRAPIE AND CELLULAR PRP ISOFORMS ARE ENCODED BY THE SAME CHROMOSOMAL GENE [J].
BASLER, K ;
OESCH, B ;
SCOTT, M ;
WESTAWAY, D ;
WALCHLI, M ;
GROTH, DF ;
MCKINLEY, MP ;
PRUSINER, SB ;
WEISSMANN, C .
CELL, 1986, 46 (03) :417-428
[4]   MASS-SPECTROMETRIC DETERMINATION OF THE AMINO-ACID-SEQUENCE OF PEPTIDES AND PROTEINS [J].
BIEMANN, K ;
MARTIN, SA .
MASS SPECTROMETRY REVIEWS, 1987, 6 (01) :1-75
[5]   A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].
CIUCANU, I ;
KEREK, F .
CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217
[6]   FAST ATOM BOMBARDMENT MASS-SPECTROMETRIC STRATEGIES FOR CHARACTERIZING CARBOHYDRATE-CONTAINING BIO-POLYMERS [J].
DELL, A ;
CARMAN, NH ;
TILLER, PR ;
THOMASOATES, JE .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 16 (1-12) :19-24
[7]   PREPARATION AND DESORPTION MASS-SPECTROMETRY OF PERMETHYL AND PERACETYL DERIVATIVES OF OLIGOSACCHARIDES [J].
DELL, A .
METHODS IN ENZYMOLOGY, 1990, 193 :647-660
[8]   A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].
DOMON, B ;
COSTELLO, CE .
GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409
[9]  
FERGUSON MAJ, 1985, J BIOL CHEM, V260, P4547
[10]  
FERGUSON MAJ, 1988, ANNU REV BIOCHEM, V57, P285
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