EFFECTS OF SUBUNIT CROSS-LINKING ON THE PROPERTIES OF CROTOXIN

被引：0

作者：

LENNON, BW ^{[1
]}

PLUMMER, D ^{[1
]}

KAISER, II ^{[1
]}

机构：

[1] UNIV WYOMING, DEPT MOLEC BIOL, LARAMIE, WY 82071 USA

来源：

TOXICON | 1990年 / 28卷 / 06期

关键词：

D O I：

10.1016/0041-0101(90)90261-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Crotoxin was cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Cross-linked crotoxin had the expected amino-terminal amino acids, amino acid composition, behavior on SDS-PAGE and an 80% reduction of reactable lysine residues. It was also non-toxic, had reduced immunological cross-reactivity toward both poly- and monoclonal antibodies raised to the basic subunit of crotoxin and had lost >95% of its phospholipase activity. Loss of toxicity is due to either subunit cross-linking or the modification of essential residues. © 1990.

引用

页码：718 / 722

页数：5