ESR SPIN TRAPPING DETECTION OF HYDROXYL RADICALS IN THE REACTIONS OF CR(V) COMPLEXES WITH HYDROGEN-PEROXIDE

被引：36

作者：

SHI, XL ^{[1
]}

DALAL, NS ^{[1
]}

机构：

[1] W VIRGINIA UNIV, DEPT CHEM, MORGANTOWN, WV 26506 USA

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 10卷 / 1-2期

关键词：

Cr(V); Cr(VI) reduction; ESR; Free radicals; Hydrogen peroxide; Spin trapping;

D O I：

10.3109/10715769009145929

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：17 / 26

页数：10

共 40 条

[11] ESR STUDY OF ELECTRONIC-STRUCTURE OF CR(V) COMPLEXES FORMED IN OXIDATION OF DIOLS BY DICHROMATE [J].

DEROUANE, EG ;

OUHADI, T .

CHEMICAL PHYSICS LETTERS, 1975, 31 (01) :70-74

[12] ELECTRON-TRANSPORT CYTOCHROME-P-450 SYSTEM IS INVOLVED IN THE MICROSOMAL METABOLISM OF THE CARCINOGEN CHROMATE [J].

GARCIA, JD ;

JENNETTE, KW .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1981, 14 (04) :281-295

[13] ELECTRON-SPIN RESONANCE STUDIES .46. OXIDATION OF THIOLS AND DISULFIDES IN AQUEOUS-SOLUTION - FORMATION OF RS., RSO., RSO2., RSSR.-, AND CARBON RADICALS [J].

GILBERT, BC ;

LAUE, HAH ;

NORMAN, ROC ;

SEALY, RC .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1975, (09) :892-900

[14] FORMATION OF CHROMIUM(V) DURING THE SLOW REDUCTION OF CARCINOGENIC CHROMIUM(VI) BY MILK AND SOME OF ITS CONSTITUENTS [J].

GOODGAME, DML ;

JOY, AM .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1987, 135 (01) :L5-L7

[15] ELECTRON-PARAMAGNETIC-RES STUDY OF THE CR(V) AND RADICAL SPECIES PRODUCED IN THE REDUCTION OF CR(VI) BY ASCORBATE [J].

GOODGAME, DML ;

JOY, AM .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1987, 135 (02) :115-118

[16] METABOLISM OF CARCINOGEN CHROMATE BY RAT-LIVER MICROSOMES [J].

GRUBER, JE ;

JENNETTE, KW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 82 (02) :700-706

[17] ELECTRON-SPIN RESONANCE STUDY OF SPIN ADDUCTS OF OH AND HO2 RADICALS WITH NITRONES IN ULTRAVIOLET PHOTOLYSIS OF AQUEOUS HYDROGEN-PEROXIDE SOLUTIONS [J].

HARBOUR, JR ;

CHOW, V ;

BOLTON, JR .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1974, 52 (20) :3549-3553

[18] SPIN TRAPPING WITH ALPHA-PYRIDYL 1-OXIDE N-TERT-BUTYL NITRONES IN AQUEOUS-SOLUTIONS - UNIQUE ELECTRON-SPIN RESONANCE-SPECTRUM FOR HYDROXYL RADICAL ADDUCT [J].

JANZEN, EG ;

WANG, YY ;

SHETTY, RV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (09) :2923-2925

[19] FACTORS INFLUENCING HYPERFINE SPLITTING IN ESR-SPECTRA OF 5-MEMBERED RING NITROXIDES [J].

JANZEN, EG ;

EVANS, CA ;

LIU, JIP .

JOURNAL OF MAGNETIC RESONANCE, 1973, 9 (03) :513-516

[20] RADICAL-ADDITION REACTIONS OF 5,5-DIMETHYL-1-PYRROLINE-1-OXIDE - ESR SPIN TRAPPING WITH A CYCLIC NITRONE [J].

JANZEN, EG ;

LIU, JIP .

JOURNAL OF MAGNETIC RESONANCE, 1973, 9 (03) :510-512

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