FLUOROMETRIC ASSAY OF ENZYMATIC REACTIONS INVOLVING ACETYL COENZYME A IN ALDOL CONDENSATIONS

被引：31

作者：

CALVO, JM

BARTHOLO.JC

STIEGLIT.BI

机构：

[1] Department of Biochemistry and Molecular Biology, Cornell University, Ithaca

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(69)90168-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis and properties (NMR, UV absorption, fluorescence emission spectra, and fluorescence quantum yield) of umbelliferone, 4-isopropylumbelliferone, and 4-carboxymethylumbelliferone are described. The optimal conditions for the formation of these umbelliferones from substituted malic acids and resorcinol have been determined. This forms the basis for a rapid and sensitive fluoremetric assay of enzymes catalyzing aldol condensation reactions involving acetyl coenzyme A. © 1969.

引用

页码：164 / &

共 31 条

[1]

ADAMS R, 1953, ORGANIC REACTIONS ED, V7, P1

[2] SPECTROPHOTOMETRIC ASSAY FOR SULFHYDRYL GROUPS USING N-ETHYLMALEIMIDE [J].