SEQUENCE-ANALYSIS OF THE CELLULASE-ENCODING CELY GENE OF ERWINIA-CHRYSANTHEMI - A POSSIBLE CASE OF INTERSPECIES GENE-TRANSFER

被引：40

作者：

GUISEPPI, A ^{[1
]}

AYMERIC, JL ^{[1
]}

CAMI, B ^{[1
]}

BARRAS, F ^{[1
]}

CREUZET, N ^{[1
]}

机构：

[1] CNRS,CHIM BACTERIENNE LAB,31 CHEMIN JOSEPH AIGUIER,BP 71,F-13277 MARSEILLE 9,FRANCE

来源：

GENE | 1991年 / 106卷 / 01期

关键词：

RECOMBINANT DNA; PHYTOPATHOGENIC BACTERIA; ENDOGLUCANASE; NUCLEOTIDE SEQUENCE; NTRA PROMOTER; HORIZONTAL TRANSFER;

D O I：

10.1016/0378-1119(91)90573-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Erwinia chrysanthemi (strain 3937) celY gene encoding the minor endoglucanase (EGY) was sequenced. The analysis of the upstream region allowed us to identify an in vivo active promoter recognized by the NtrA (sigma-54) holoenzyme. No similarity was found between the predicted amino acid (aa) sequences of EGY and either the Er. chrysanthemi major endoglucanase, EGZ, or the Er. carotovora CelS endoglucanase. In contrast, a very high level of identity, both at the nucleotide and the predicted aa levels, was found between celY and an EG-encoding gene from Cellulomonas uda, a Gram+ bacterium taxonomically distant from Er. chrysanthemi. By comparing the molar G + C% of the cellulase-encoding genes and that of Er. chrysanthemi and C. uda chromosomal DNAs, we speculate that celY was transferred from Er. chrysanthemi to C. uda.

引用

页码：109 / 114

页数：6

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