ABSORPTION EFFECTS ON TOTAL-INTERNAL-REFLECTION FLUORESCENCE SPECTROSCOPY

被引:20
作者
TORIUMI, M
YANAGIMACHI, M
MASUHARA, H
机构
[1] RES DEV CORP JAPAN,EXPLORATORY RES ADV TECHNOL PROGRAM,MICROPHOTOCONVERS PROJECT,SAKYO KU,KYOTO 606,JAPAN
[2] OSAKA UNIV,DEPT APPL PHYS,SUITA,OSAKA 565,JAPAN
来源
APPLIED OPTICS | 1992年 / 31卷 / 30期
关键词
ABSORPTION; TOTAL INTERNAL REFLECTION; FLUORESCENCE SPECTROSCOPY;
D O I
10.1364/AO.31.006376
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A theoretical consideration is made for transverse-electric polarization vectors of a transmitted field in an optically absorbing sample to derive a fluorescence intensity formula as a function of the absorption index of the sample as well as incident and observation angles in total-internal-reflection fluorescence spectroscopy. These equations are experimentally confirmed. It is shown that the angular spectrum of fluorescence intensity is the Laplace transformation of the effective penetration depth, even for the absorbing sample. High resolution of the depth profile is expected for an absorbing sample compared with a nonabsorbing one.
引用
收藏
页码:6376 / 6382
页数:7
相关论文
共 50 条
[31]   Fluorescence photobleaching recovery using total internal reflection interference fringes [J].
Hagen, Guy M. ;
Roess, Deborah A. ;
Barisas, B. George .
ANALYTICAL BIOCHEMISTRY, 2006, 356 (01) :30-35
[32]   The influence of polarized light on fluorescence emission in total internal reflection microscopy [J].
Wang, C ;
Yuan, JH ;
Wang, GY ;
Xu, ZZ .
ACTA PHYSICA SINICA, 2003, 52 (12) :3014-3019
[33]   Total Internal Reflection Fluorescence Microscopy for Investigation of Dynamics of Single Molecules [J].
Savchenko, Ekaterina ;
Kuznetsova, Olga .
PROCEEDINGS OF THE 2018 IEEE INTERNATIONAL CONFERENCE ON ELECTRICAL ENGINEERING AND PHOTONICS (EEXPOLYTECH), 2018, :268-271
[34]   Time-gated total internal reflection fluorescence spectroscopy (TG-TIRFS): application to the membrane marker laurdan [J].
Schneckenburger, H ;
Stock, K ;
Strauss, WSL ;
Eickholz, J ;
Sailer, R .
JOURNAL OF MICROSCOPY, 2003, 211 (211) :30-36
[35]   Total Internal Reflection-Based Extinction Spectroscopy of Single Nanoparticles [J].
Li, Meng ;
Yuan, Tinglian ;
Jiang, Yingyan ;
Sun, Linlin ;
Wei, Wei ;
Chen, Hong-Yuan ;
Wang, Wei .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2019, 58 (02) :572-576
[36]   Total internal reflection fluorescence lifetime and anisotropy screening of cell membrane dynamics [J].
Bruns, Thomas ;
Strauss, Wolfgang S. L. ;
Schneckenburger, Herbert .
JOURNAL OF BIOMEDICAL OPTICS, 2008, 13 (04)
[37]   Measurement of small wavelength shift using diffraction grating and high-angular-sensitivity total-internal-reflection heterodyne interferometer [J].
Lin, Jiun-You ;
Jhuang, Jing-Hsiang ;
Hsieh, Meng-Chang ;
Chang, Chia-Ou .
OPTICS AND LASERS IN ENGINEERING, 2018, 100 :155-160
[38]   Adsorption of a protein-porphyrin complex at a liquid-liquid interface studied by total internal reflection synchronous fluorescence spectroscopy [J].
Tang, Yao-Ji ;
Chen, Ying ;
Chen, Zhe ;
Xie, Tang-Tang ;
Li, Yao-Qun .
ANALYTICA CHIMICA ACTA, 2008, 614 (01) :71-76
[39]   DEPTH PROFILE OF PYRENE DOPANT IN POLYMER-FILMS BY VARIABLE-ANGLE TOTAL INTERNAL-REFLECTION FLUORESCENCE SPECTROSCOPY [J].
YANAGIMACHI, M ;
TORIUMI, M ;
MASUHARA, H .
APPLIED SPECTROSCOPY, 1992, 46 (05) :832-840
[40]   Polymer 1x2 Thermo-Optic Digital Optical Switch Based on the Total-Internal-Reflection Effect [J].
Han, Young-Tak ;
Shin, Jang-Uk ;
Park, Sang-Ho ;
Han, Sang-Pil ;
Baek, Yongsoon ;
Lee, Chul-Hee ;
Noh, Young-Ouk ;
Park, Hyo-Hoon .
ETRI JOURNAL, 2011, 33 (02) :275-278