ABSORPTION EFFECTS ON TOTAL-INTERNAL-REFLECTION FLUORESCENCE SPECTROSCOPY

被引:20
作者
TORIUMI, M
YANAGIMACHI, M
MASUHARA, H
机构
[1] RES DEV CORP JAPAN,EXPLORATORY RES ADV TECHNOL PROGRAM,MICROPHOTOCONVERS PROJECT,SAKYO KU,KYOTO 606,JAPAN
[2] OSAKA UNIV,DEPT APPL PHYS,SUITA,OSAKA 565,JAPAN
来源
APPLIED OPTICS | 1992年 / 31卷 / 30期
关键词
ABSORPTION; TOTAL INTERNAL REFLECTION; FLUORESCENCE SPECTROSCOPY;
D O I
10.1364/AO.31.006376
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A theoretical consideration is made for transverse-electric polarization vectors of a transmitted field in an optically absorbing sample to derive a fluorescence intensity formula as a function of the absorption index of the sample as well as incident and observation angles in total-internal-reflection fluorescence spectroscopy. These equations are experimentally confirmed. It is shown that the angular spectrum of fluorescence intensity is the Laplace transformation of the effective penetration depth, even for the absorbing sample. High resolution of the depth profile is expected for an absorbing sample compared with a nonabsorbing one.
引用
收藏
页码:6376 / 6382
页数:7
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