PLEIOTROPHIN TRANSFORMS NIH 3T3 CELLS AND INDUCES TUMORS IN NUDE-MICE

被引：140

作者：

CHAUHAN, AK

LI, YS

DEUEL, TF

机构：

[1] WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT MED,216 S KINGSHIGHWAY,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,JEWISH HOSP ST LOUIS,MED CTR,DEPT BIOCHEM & MOLEC BIOPHYS,ST LOUIS,MO 63110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 02期

关键词：

PROTOONCOGENE; GROWTH FACTOR; TRANSFORMATION;

D O I：

10.1073/pnas.90.2.679

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pleiotrophin (PTN) gene (Ptn) encodes an 18-kDa protein that is highly conserved among mammalian species and that functions as a weak mitogen and promotes neurite-outgrowth activity in vitro. To further investigate the role PTN plays in regulating cell growth, we overexpressed the bovine PTN cDNA and now show that PTN phenotypically transforms NIH 3T3 cells, as evidenced by increased cell number at confluence, focus formation, anchorage-independent growth, and tumor formation in the nude mouse. The results demonstrate that the Ptn gene has the potential to regulate NIH 3T3 cell growth and suggest that PTN may influence abnormal cell growth in vivo.

引用

页码：679 / 682

页数：4

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