THERMODYNAMIC STUDY OF LANGMUIR ANTIBODY FILMS FOR APPLICATION TO IMMUNOSENSORS

被引：37

作者：

AHLUWALIA, A ^{[1
]}

DEROSSI, D ^{[1
]}

MONICI, M ^{[1
]}

SCHIRONE, A ^{[1
]}

机构：

[1] ENTE NAZL ENERGIA ATOM,CTR RIC ENERGIA AMBIENTE,I-19100 LA SPEZIA,ITALY

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 02期

关键词：

LANGMUIR-BLODGETT FILMS; IMMUNOGLOBULIN-G; TOTAL INTERNAL REFLECTION FLUORESCENCE; IMMUNOSENSOR;

D O I：

10.1016/0956-5663(91)87037-C

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The thermodynamic functions, compressibility, entropy and free energy of desorption have been derived from a set of pi-A isotherms of human immunoglobulin G films at an air-water interface. Criteria for optimum filmlift conditions have been obtained from an analysis of these functions. By the application of a basic model, a picture of the desorption behaviour of segments of the antibodies has also been constructed. The results of this study have been used to estimate the theoretical sensing limit of a TIRF based immunosensor.

引用

页码：133 / 141

页数：9

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