HUMAN CYTOMEGALOVIRUS VIREMIA IN HIV-1-SEROPOSITIVE PATIENTS AT VARIOUS CLINICAL STAGES OF INFECTION

被引：78

作者：

GERNA, G ^{[1
]}

PAREA, M ^{[1
]}

PERCIVALLE, E ^{[1
]}

ZIPETO, D ^{[1
]}

SILINI, E ^{[1
]}

BARBARINI, G ^{[1
]}

MILANESI, G ^{[1
]}

机构：

[1] CNR,IST GENET BIOCHIM & EVOLUZIONIST,PAVIA,ITALY

来源：

AIDS | 1990年 / 4卷 / 10期

关键词：

HCMV immediate-early antigens; HCMV viraemia; HIV-1; infection; human cytomegalovirus; immunocompromised; immunofluorescence; monoclonal antibodies; polymerase chain reaction;

D O I：

10.1097/00002030-199010000-00014

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Eighty-two HIV-1-seropositive subjects were examined for the presence and quantification of human cytomegalovirus (HCMV) in peripheral blood polymorphonuclear leukocytes (PMNL) by polymerase chain reaction, culture and immunofluorescence in order to investigate the relationship between viraemia and immunosuppression. Patients were divided into three groups: (1) asymptomatic subjects with > 400 x 106/l CD4 lymphocytes (n = 30); (2) asymptomatic subjects with < 400 x 106/l of CD4 lymphocytes and zidovudine (n = 20), and (3) AIDS-related complex (ARC)/AIDS patients on zidovudine (n = 32). Evidence of HCMV infection in circulating PMNL was found in 15 out of 29 ARC/AIDS patients examined (51.7%), whereas no infection was detected among the 50 asymptomatic HIV-1-seropositive subjects. HCMV-related symptoms were found only where the number of infected PMNL was > 50 per 2 x 105 cells.

引用

页码：1027 / 1031

页数：5

共 24 条

[1] THE STRUCTURE OF THE MAJOR IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS STRAIN AD169 [J].