MODULAR STRUCTURE OF A CHICKEN LYSOZYME SILENCER - INVOLVEMENT OF AN UNUSUAL THYROID-HORMONE RECEPTOR-BINDING SITE

被引：444
作者：
BANIAHMAD, A
STEINER, C
KOHNE, AC
RENKAWITZ, R
机构：
[1] Genzentrum Max-Planck-Institut für Biochemie
来源：
CELL | 1990年 / 61卷 / 03期
关键词：
D O I：
10.1016/0092-8674(90)90532-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Silencer elements, by analogy to enhancer elements, function independently of their position and orientation. We show that the chicken lysozyme silencer S-2.4 kb has many other characteristics in common with enhancer elements. The silencer is comprised of modules that independently repress gene activity-repression being increased synergistically when different or identical modules are combined. Repression is effective both on a complete and on a minimal promoter consisting of a TATA box only. One silencer module is bound in vitro by a 75-93 kd protein, termed NeP1; the other can be bound either by the product of the oncogene v-erbA or by the thyroid hormone receptor. This erbA binding site is unusual in that the palindromic sequence is inverted. © 1990.
引用
收藏
页码：505 / 514
页数：10
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