AN EVALUATION OF THE INACTIVE MOUSE X-CHROMOSOME IN SOMATIC-CELL HYBRIDS

被引：7
作者：
SALIDO, EC
PASSAGE, MB
YEN, PH
SHAPIRO, LJ
MOHANDAS, TK
机构：
[1] UCLA,HARBOR MED CTR,DIV MED GENET,TORRANCE,CA 90509
[2] UNIV LA LAGUNA,FAC MED,DEPT PATHOL,LA LAGUNA,SPAIN
来源：
SOMATIC CELL AND MOLECULAR GENETICS | 1993年 / 19卷 / 01期
关键词：
D O I：
10.1007/BF01233955
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamster-mouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F1 females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.
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收藏
页码：65 / 71
页数：7
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