OXIDATION OF LOW-DENSITY-LIPOPROTEIN BY THIOLS - SUPEROXIDE-DEPENDENT AND SUPEROXIDE-INDEPENDENT MECHANISMS

Oxidatively damaged low density lipoprotein (LDL) may cause macrophages to accumulate cholesterol in an unregulated manner, initiating the development of atherosclerotic lesions. Cultured smooth muscle cells oxidize LDL by a superoxide (O2-)-dependent mechanism that requires L-cystine and redox-active transition metal ions in the incubation medium. To test the hypothesis that cellular reduction of L-cystine to a thiol might be involved, we exposed LDL to L-cysteine, glutathione, and D,L-homocysteine. In a cell-free system each thiol modified LDL by a pathway that required either Cu2+ or Fe3+. Thiol- and Cu2+-modified LDL underwent lipid peroxidation and exhibited a number of properties of cell-modified LDL, including increased mobility on agarose gel electrophoresis and fragmentation of apolipoprotein B-100. Superoxide dismutase inhibited modification of LDL by L-cysteine/Cu2+ whereas catalase and mannitol were without effect. In striking contrast, superoxide dismutase had little effect on oxidation of LDL by CU2+ and either homocysteine or glutathione. Moreover, only L-cysteine/CU2+-modified I-125-labeled LDL was degraded more rapidly than I-125-labeled LDL by human monocyte-derived macrophages: superoxide dismutase in the reaction mixture blocked the facilitated uptake of L-cysteine/Cu2+-Modified I-125-labeled LDL, suggesting involvement Of O2-. These results indicate that LDL oxidation by L-cysteine and CU2+ requires O2- but not H2O2 or hydroxyl radical. The reaction may involve the metal ion-dependent formation of L-cystine radical anion which is oxidized by oxygen, yielding O2- and the disulfide. LDL modified by L-cysteine and smooth muscle cells exhibit similar physical and biological properties, indicating that thiol-dependent generation of O2- may be the oxidative mechanism in both systems. Thiols also promote lipid peroxidation by O2--independent reactions but human macrophages fail to rapidly degrade these oxidized LDLs.