TRANSPLANTATION OF FETAL NIGRAL CELLS REVERSES THE INCREASE OF PREPROENKEPHALIN MESSENGER-RNA LEVELS IN THE RAT STRIATUM CAUSED BY 6-OHDA LESION OF THE DOPAMINERGIC NIGROSTRIATAL PATHWAY - A QUANTITATIVE INSITU HYBRIDIZATION STUDY

被引：22
作者：
BAL, A
SAVASTA, M
CHRITIN, M
MENNICKEN, F
ABROUS, DN
LEMOAL, M
FEUERSTEIN, C
HERMAN, JP
机构：
[1] CHU GRENOBLE,DEPT NEUROSCI CLIN & BIOL,LAPSEN,INSERM,U318,PAVILLON NEUROL,BP 217,F-38043 GRENOBLE 9,FRANCE
[2] INSERM,U259,F-33077 BORDEAUX,FRANCE
来源：
MOLECULAR BRAIN RESEARCH | 1993年 / 18卷 / 03期
关键词：
PREPROENKEPHALIN-A MESSENGER RNA; NIGROSTRIATAL DOPAMINE PATHWAY; INTRACEREBRAL NEURAL TRANSPLANTATION; INSITU HYBRIDIZATION; AUTORADIOGRAPHY;
D O I：
10.1016/0169-328X(93)90193-S
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Unilateral 6-hydroxydopamine (6-OHDA)-induced lesion of the nigrostriatal dopamine (DA) pathway causes a significant increase of preproenkephalin (PPE) messenger RNA (mRNA) levels in the DA-depleted striatum in rat brain. Using an in situ hybridization (ISH) technique and computer-assisted microdensitometry, we quantified the changes in PPE MRNA levels in the striatum. Seven months after lesion, levels of PPE mRNA were 75% higher in the DA-depleted striatum than in the contralateral control striatum of the same animal or in the striatum of sham control animals. The implantation of embryonic dopaminergic neurons into the denervated striatum led to a complete reversal of this increase and, in grafted animals, levels of PPE mRNA were at control values. Moreover, this reversal extended beyond the areas reinnervated by the grafted dopaminergic neurons.
引用
收藏
页码：221 / 227
页数：7
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