CELLS FROM EMBRYONIC RAT STRIATUM COCULTURED WITH MESENCEPHALIC GLIA EXPRESS DOPAMINERGIC PHENOTYPES

被引：18

作者：

BEYER, C ^{[1
]}

PILGRIM, C ^{[1
]}

REISERT, I ^{[1
]}

MISGELD, U ^{[1
]}

机构：

[1] UNIV HEIDELBERG,INST PHYSIOL,W-6900 HEIDELBERG,GERMANY

来源：

NEUROSCIENCE LETTERS | 1991年 / 128卷 / 01期

关键词：

COCULTURE; DOPAMINE; IMMUNOCYTOCHEMISTRY; NEOSTRIATUM; SUBSTANTIA NIGRA; TYROSINE HYDROXYLASE; RAT; IMMUNOREACTIVE NEURONS; HETEROGENEITY; BRAIN;

D O I：

10.1016/0304-3940(91)90746-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To study region-specific transmitter phenotype expression, cells of embryonic day 14 (E14) rat neostriatum (ganglionic eminence plus cortical plate) or of the substantia nigra (ventral mesencephalon) were cultured on glial cells either from substantia nigra or neostriatum (E21). By anti-tyrosine hydroxylase immunocytochemistry, immunoblotting of tyrosine hydroxylase protein and quantitation of dopamine and its metabolites by HPLC, dopaminergic cells were revealed in nigral and neostriatal cultures plated on nigral glial cells. No dopaminergic cells were found among neostriatal neurons plated on neostriatal glial cells. It is concluded that glia from substantia nigra but not glia from neostriatum is capable of inducing development or promoting survival of dopaminergic cells.

引用

页码：1 / 3

页数：3

共 15 条

[1] REGION-SPECIFIC NEURO-ASTROGLIAL INTERACTIONS - ULTRASTRUCTURAL-STUDY OF THE INVITRO EXPRESSION OF NEURONAL POLARITY [J].