CONCENTRATION-DEPENDENT EFFECTS OF DENTIN PHOSPHORYN IN THE REGULATION OF INVITRO HYDROXYAPATITE FORMATION AND GROWTH

被引：152

作者：

BOSKEY, AL ^{[1
]}

MARESCA, M ^{[1
]}

DOTY, S ^{[1
]}

SABSAY, B ^{[1
]}

VEIS, A ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,SCH DENT MED,CHICAGO,IL 60611

来源：

BONE AND MINERAL | 1990年 / 11卷 / 01期

关键词：

Dentin; Dentin phosphoprotein; Hydroxyapatite; Mineralization; Phosphophoryn;

D O I：

10.1016/0169-6009(90)90015-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The effect of dentin phosphophoryn on hydroxyapatite formation and growth was studied in an in vitro gelatin gel diffusion system. Phosphophoryn, in low concentrations (0.010-1 μg/ml) promoted de novo hydroxyapatite formation; at a higher concentration (100 μg/ml) in the same system, the dentin matrix protein inhibited hydroxyapatite growth. Similar inhibition of hydroxyapatite growth was seen in solution. The intact phosphophoryn was not essential for either inhibition of seeded growth or promotion of mineralization, since the formic acid degraded protein was comparably effective. Transmission electron microscopy of the precipitates formed at 7 days showed no significant differences in crystallite size distribution in the presence and absence of phosphophoryn. However there was a dose-dependent decrease in the number of mineral clusters formed in the presence of increasing amounts of phosphophoryn, suggesting inhibition of secondary nucleation. These data provide support for the postulated 'multifunctional' role of the dentin phosphoprotein in the mineralization process. © 1990.

引用

页码：55 / 65

页数：11

共 38 条

[1] FORMATION AND STRUCTURE OF CA-DEFICIENT HYDROXYAPATITE [J].