NOVEL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD USING SOLID-PHASE ONLINE ELUTION FOR DETERMINATION OF METOLAZONE IN PLASMA AND WHOLE-BLOOD

被引：17

作者：

FARTHING, D

SICA, DA

FAKHRY, I

GEHR, TWB

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DIV CLIN PHARMACOL,MCV STN BOX 160,RICHMOND,VA 23298

[2] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DIV HYPERTENS & NEPHROL,RICHMOND,VA 23298

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 653卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0427-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel solid-phase on-line elution HPLC method employing fluorescence detection to measure metolazone in plasma and whole blood has been developed. The method is sensitive and selective for metolazone and linear over a dynamic range of 1-50 ng/ml with a sample requirement of 250 mul. The limit of quantitation for the method is 1 ng/ml and combined intra- and inter-day accuracy and precision had an error and coefficient of variation of 2.9 and 5.5%, respectively.

引用

页码：171 / 176

页数：6

共 7 条

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