RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

被引：0

作者：

Yan, Shijun ^{[1
]}

Yan, He ^{[1
]}

Zhang, Chaolin ^{[1
]}

Wang, Tongyan ^{[1
]}

Yang, Qingyuan ^{[1
]}

Sun, Zhe ^{[1
]}

Xiao, Yan ^{[1
]}

Tan, Feifei ^{[1
]}

Li, Xiangdong ^{[1
]}

Tian, Kegong ^{[1
,2
]}

机构：

[1] Natl Res Ctr Vet Med, Luoyang 471003, Peoples R China

[2] Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

来源：

FRONTIERS OF AGRICULTURAL SCIENCE AND ENGINEERING | 2016年 / 3卷 / 01期

关键词：

pseudorabies virus; RR1 and RR2; ribonucleotide reductase; vaccine candidate;

D O I：

10.15302/J-FASE-2016088

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/gE/gI triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

引用

页码：81 / 86

页数：6

共 14 条

[1] Repair of the UL21 Locus in Pseudorabies Virus Bartha Enhances the Kinetics of Retrograde, Transneuronal Infection In Vitro and In Vivo
Curanovic, D.
Lyman, M. G.
Bou-Abboud, C.
Card, J. P.
Enquist, L. W.
[J]. JOURNAL OF VIROLOGY, 2009, 83 (03) : 1173 - 1183
[2] RIBONUCLEOTIDE REDUCTASE-DEFICIENT MUTANTS OF PSEUDORABIES VIRUS ARE AVIRULENT FOR PIGS AND INDUCE PARTIAL PROTECTIVE IMMUNITY
DEWIND, N
BERNS, A
GIELKENS, A
KIMMAN, T
[J]. JOURNAL OF GENERAL VIROLOGY, 1993, 74 : 351 - 359
[3] MUTAGENESIS AND CHARACTERIZATION OF A 41-KILOBASE-PAIR REGION OF THE PSEUDORABIES VIRUS GENOME - TRANSCRIPTION MAP, SEARCH FOR VIRULENCE GENES, AND COMPARISON WITH HOMOLOGS OF HERPES-SIMPLEX VIRUS TYPE-1
DEWIND, N
PEETERS, BPH
ZIJDERVELD, A
GIELKENS, ALJ
BERNS, AJM
KIMMAN, TG
[J]. VIROLOGY, 1994, 200 (02) : 784 - 790
[4] HERPES-SIMPLEX VIRUS TYPE-1-INDUCED RIBONUCLEOTIDE REDUCTASE-ACTIVITY IS DISPENSABLE FOR VIRUS GROWTH AND DNA-SYNTHESIS - ISOLATION AND CHARACTERIZATION OF AN ICP6 LACZ INSERTION MUTANT
GOLDSTEIN, DJ
WELLER, SK
[J]. JOURNAL OF VIROLOGY, 1988, 62 (01) : 196 - 205
[5] A novel inactivated gE/gI deleted pseudorabies virus (PRV) vaccine completely protects pigs from an emerged variant PRV challenge
Gu, Zhenqing
Dong, Jing
Wang, Jichun
Hou, Chengcai
Sun, Haifeng
Yang, Wenping
Bai, Juan
Jiang, Ping
[J]. VIRUS RESEARCH, 2015, 195 : 57 - 63
[6] LARGE AND SMALL SUBUNITS OF THE AUJESZKYS-DISEASE VIRUS RIBONUCLEOTIDE REDUCTASE - NUCLEOTIDE-SEQUENCE AND PUTATIVE STRUCTURE
KALIMAN, AV
BOLDOGKOI, Z
FODOR, I
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1219 (01): : 151 - 156
[7] METTENLEITER TC, 1994, ACTA VET HUNG, V42, P153
[8] Immunobiology of pseudorabies (Aujeszky's disease)
Mettenleiter, TC
[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 54 (1-4) : 221 - 229
[9] Molecular biololgy of pseudorabies virus: Impact on neurovirology and veterinary medicine
Pomeranz, LE
Reynolds, AE
Hengartner, CJ
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2005, 69 (03) : 462 - +
[10] THE HERPES-SIMPLEX VIRUS TYPE-1 TEMPERATURE-SENSITIVE MUTANT TS1222 HAS A SINGLE BASE PAIR DELETION IN THE SMALL SUBUNIT OF RIBONUCLEOTIDE REDUCTASE
PRESTON, VG
DARLING, AJ
MCDOUGALL, IM
[J]. VIROLOGY, 1988, 167 (02) : 458 - 467

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