A MICROSCALE METHOD FOR COLORIMETRIC DETERMINATION OF UREA IN SOIL EXTRACTS

被引：29

作者：

GREENAN, NS ^{[1
]}

MULVANEY, RL ^{[1
]}

SIMS, GK ^{[1
]}

机构：

[1] USDA ARS,URBANA,IL 61801

来源：

COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS | 1995年 / 26卷 / 15-16期

关键词：

D O I：

10.1080/00103629509369465

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100-mu L aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate ia a low-temperature oven at 87 degrees C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.

引用

页码：2519 / 2529

页数：11

共 10 条

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