EVIDENCE FOR AN INHIBITOR IN CONTROL OF GLOBIN SYNTHESIS BY HEMIN IN A RETICULOCYTE LYSATE

被引：108

作者：

MAXWELL, CR

RABINOVITZ, M

机构：

[1] Laboratory of Physiology, National Cancer Institute, National Institutes of Health, Bethesda, MD

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 35卷 / 01期

关键词：

D O I：

10.1016/0006-291X(69)90485-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of hemoglobin by a reticulocyte lysate is stimulated several fold by the addition of hemin. The ability to respond to hemin is completely lost if the lysate is held for a short time at 34°C, but only slightly reduced if held at 0°C. Hemoglobin synthesis by reconstituted lysates prepared by combinations of ribosomal and supernatant fractions from hemin-responsive and -unresponsive lysates indicated that the factor mediating hemin stimulation is in the supernatant fluid. Addition of supernatant fraction from an unresponsive lysate to an incorporating system containing a responsive lysate markedly decreased the stimulation of globin synthesis by hemin. These results suggest that hemin promotes globin synthesis by preventing the formation of an inhibitor of chain initiation in the cell sap. © 1969.

引用

页码：79 / +

页数：1

共 19 条

[1]

ABERCROMBIE M, 1967, ADVANCES MORPHOGE ED, V6, P89

[2] FACTORS AFFECTING RATE OF PROTEIN SYNTHESIS IN LYSATE SYSTEMS FROM RETICULOCYTES [J].