A RADIOCHEMICAL METHOD FOR MONOAMINE OXIDASE ASSAY

被引：8
作者：
FELLMAN, JH
ROTH, ES
MOLLICA, RF
机构：
[1] Department of Biochemistry, Division of Neurology, University of Oregon Medical School, Portland
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 03期
关键词：
D O I：
10.1016/0003-2697(69)90126-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple radiochemical method is described for the assay of monoamine oxidase utilizing tritiated tyramine. The synthesis of tyramine-1,2-3H from tyrosine-2,3-3H is reported. Tritium, released by the oxidation of tyramine-1,2-3H by monoamine oxidase, was counted by conventional liquid scintillation techniques. Also reported is the application of this method for the study of a number of parameters which influence the activity of the enzyme, including pH, substrate concentration, and amount of enzyme. © 1969.
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页码：339 / &
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