PROPERTIES AND PURIFICATION OF A GLUCOSE-REGULATED PROTEIN FROM CHICK-EMBRYO FIBROBLASTS

被引：31

作者：

SHIU, RPC ^{[1
]}

PASTAN, IH ^{[1
]}

机构：

[1] NCI,MOLEC BIOL LAB,BETHESDA,MD 20014

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 576卷 / 01期

关键词：

(Fibroblast; Rous sarcoma virus); Glucose; Immunofluorescence; Transformation;

D O I：

10.1016/0005-2795(79)90493-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A glucose-regulated protein of molecular weight 78 000 (GRP-78) had been purified from a membrane fraction isolated from viral transformed chick embryo fibroblasts. Purification was achieved by extraction of the membrane fraction with Triton X-100, and chromatography on diethylaminoethyl-cellulose and hydroxyapatite. The purified protein exhibited one single spot on two-dimensional polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and has a pI of about 5.3. A monospecific antiserum to GRP-78 was generated in a goat. Immunofluorescence studies using affinity purified antibodies to GRP-78 revealed that this protein was not exposed on the cell surface but was localized in a granular vesicular network inside the cell that resembles the distribution of endoplasmic reticulum. The availability of purified GRP-78 and a specific antiserum to it should prove useful in elucidating the role of this protein in glucose metabolism and its relationship to malignant transformation. © 1979.

引用

页码：141 / 150

页数：10

共 16 条

[1] TEMPERATURE-DEPENDENT TRANSFORMATION OF CELLS INFECTED WITH A MUTANT OF BRYAN ROUS-SARCOMA VIRUS [J].