DIRECT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION IN URINE OF THE ENANTIOMERS OF PROPRANOLOL AND ITS MAJOR BASIC METABOLITE 4-HYDROXYPROPRANOLOL

被引：27

作者：

HERRING, VL ^{[1
]}

JOHNSON, JA ^{[1
]}

机构：

[1] UNIV TENNESSEE CTR HLTH SCI,COLL PHARM,DEPT CLIN PHARM,26 S DUNLAP ST,SUITE 200,MEMPHIS,TN 38163

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 612卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80166-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for quantitation of underivatized enantiomers of propranolol and its major basic metabolite, 4-hydroxypropranolol, in urine samples by high-performance liquid chromatography with fluorescence detection, using a cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase. This method was found to be precise and accurate for the measurement of propranolol and 4-hydroxypropranolol concentrations in urine from pharmacokinetic investigations. This method represents the first assay for direct determination of 4-hydroxypropranolol enantiomers. The ability to easily measure 4-hydroxypropranolol enantiomers is valuable because the stereoselective disposition of propranolol is primarily due to stereoselective metabolism in the pathway responsible for generation of 4-hydroxypropranolol.

引用

页码：215 / 221

页数：7

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