INVESTIGATIONS OF THE PROTEIN DIGESTIBILITY AND THE ABSORPTION OF AMINO-ACIDS IN VARIOUS SEGMENTS OF THE DIGESTIVE-TRACT OF PIGS .3. RESULTS OF THE FRACTIONING OF THE ILEUM (CHYME AFTER FEEDING VARIOUS RATIONS)

Growing female pigs provided with re-entrant ileum resp. ileocecal cannulae received 6 different rations (fattening feed I and II for pigs, rations with dried skim milk, wheat gluten + lysine resp. wheat + wheat gluten + lysine, N-free mixture). The ileum chyme was separated into the fractions supernatant 1 and sediment 1 and by a treatment of supernatant 1 with trichloracetic acid and subsequent centrifuging into supernatant 2 and sediment 2. In the complete chyme as well as in all fractions the crude protein and amino acid contents were determined. The distribution in per cent of the crude protein and the amino acids over the fractions was calculated as well. The influence of the rations, in particular rations of dried skim milk, on the amino acid pattern of the complete chyme and the chyme fractions could be established. An influence of the rations could not be recognised on sediment 2 only, which indicates a uniform amino acid pattern of these probably endogenous proteins. The percentage of crude protein and amino acids in the fractions was also influenced by the rations.