N-15 NMR-SPECTROSCOPY OF PROTEINS IN SOLUTION

被引：13
|
作者：
BOGUSKY, MJ
LEIGHTON, P
SCHIKSNIS, RA
KHOURY, A
LU, P
OPELLA, SJ
机构：
[1] Department of Chemistry, University of Pennsylvania, Philadelphia
来源：
JOURNAL OF MAGNETIC RESONANCE | 1990年 / 86卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/0022-2364(90)90207-P
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Experimental results from 15N NMR studies of selectively and uniformly labeled proteins with molecular weights ranging between 6000 and 155,000 Da in solution are described. These results demonstrate the resolution available in one- and two-dimensional 15N NMR spectra as well as the selection of particular 15N and 1H resonances on the basis of the type of amino acid residue, the number of hydrogens bonded to a nitrogen, local dynamics, and amide hydrogen exchange kinetics. © 1990.
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页码：11 / 29
页数：19
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