AUTOMATED, CONTINUOUS ANALYSIS FOR CONCENTRATION OF TOTAL CARBOHYDRATE AND REDUCING END-GROUP IN FRACTIONATION OF DEXTRAN ON POROUS SILICA BEADS

The use of automated, continuous analyses for the concentration of total carbohydrate and reducing end-group to monitor the eluate in the fractionation of samples of dextran on porous silica beads is described. The two elution curves obtained were use to plot a calibration graph for the fractionation of that particular sample, and hence enabled us to make a calculation of the molecular weight distribution. Significant deviations from the S-shaped curve, used previously as the calibration graph, were noted. The mutual interaction of the molecules in the polysaccharide sample is suggested to account for these effects. © 1969.