CHOLESTEROL CONDENSATION OF ALPHA-LINOLENIC AND GAMMA-LINOLENIC ACID-CONTAINING PHOSPHATIDYLCHOLINE MONOLAYERS AND BILAYERS

被引：20

作者：

STILLWELL, W ^{[1
]}

EHRINGER, WD ^{[1
]}

DUMAUAL, AC ^{[1
]}

WASSALL, SR ^{[1
]}

机构：

[1] INDIANA UNIV PURDUE UNIV,DEPT PHYS,INDIANAPOLIS,IN 46202

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1214卷 / 02期

关键词：

CHOLESTEROL; FATTY ACID; N-3; N-6; LINOLENIC ACID; CHOLESTEROL CONDENSATION;

D O I：

10.1016/0005-2760(94)90036-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol is demonstrated to condense phosphatidylcholine (PC) monolayers and bilayers containing stearic acid in the sn-1 position and alpha-linolenic acid in the sn-2 position (18:0,alpha-18:3 PC) but has no effect when gamma-linolenic acid occupies the sn-2 position (18:0,gamma-18:3 PC). Cholesterol-induced condensation is measured by area/molecule determinations made on monolayers using a Langmuir trough, while condensation in bilayers is followed by the fluorescent dyes merocyanine (MC540) and dansyllysine. Permeability to erythritol is also demonstrated to be diminished by cholesterol for the condensable 18:0,alpha-18:3 PC bilayer membranes but not the 18:0,gamma-18:3 PC membranes, alpha- and gamma-linolenic acid are isomers containing 18 carbons and three unsaturations. Both fatty acids have unsaturations at positions 9 and 12 and differ only in the location of the third unsaturation, at either position 6 for gamma-linolenic acid (an omega-6 fatty acid) and at position 15 for alpha-linolenic acid (an omega-3 fatty acid). Here lipid-cholesterol interaction is used to distinguish the effect of position of unsaturation on membrane structure.

引用

页码：131 / 136

页数：6

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