INHIBITION OF PROTEIN-KINASE-C BY CALPHOSTIN-C IS LIGHT-DEPENDENT

被引：402

作者：

BRUNS, RF ^{[1
]}

MILLER, FD ^{[1
]}

MERRIMAN, RL ^{[1
]}

HOWBERT, JJ ^{[1
]}

HEATH, WF ^{[1
]}

KOBAYASHI, E ^{[1
]}

TAKAHASHI, I ^{[1
]}

TAMAOKI, T ^{[1
]}

NAKANO, H ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS, MACHIDA, TOKYO, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90922-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calphostin C, a secondary metabolite of the fungus Cladosporium cladosporioides, inhibits protein kinase C by competing at the binding site for diacylglycerol and phorbol esters. Calphostin C is a polycyclic hydrocarbon with strong absorbance in the visible and ultraviolet ranges. In characterizing the activity of this compound, we unexpectedly found that the inhibition of [3H]phorbol dibutyrate binding was dependent on exposure to light. Ordinary fluorescent light was sufficient for full activation. The inhibition of protein kinase C activity in cell-free systems and intact cells also required light. Light-dependent cytotoxicity was seen at concentrations about 5-fold higher than those inhibiting protein kinase C. © 1991.

引用

页码：288 / 293

页数：6

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