EXPRESSION OF RAT RENAL SODIUM-PHOSPHATE COTRANSPORTER IN XENOPUS-LAEVIS OOCYTES

被引：0
作者：
ALMAHROUQ, HA
KEMPSON, SA
机构：
[1] Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, IN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1104卷 / 01期
关键词：
EXPRESSION; PHOSPHATE TRANSPORT; MESSENGER RNA; BRUSH-BORDER MEMBRANE; OOCYTE; (RAT KIDNEY);
D O I：
10.1016/0005-2736(92)90134-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In an attempt to identify the renal Na+/P(i) cotransporter, Xenopus laevis oocytes were used to express mRNA isolated from the renal cortex of rat kidney. Na+-dependent uptake of P(i) in oocytes, injected with mRNA, resulted in an increase of 2-4-fold as compared to oocytes injected with water. Both the new expressed and endogenous Na+-dependent P(i) uptake activity were inhibited with 2 mM phosphonoformic acid (PFA). Expression of P(i) uptake into oocytes was dose-dependent with the amount of mRNA injected. When mRNA was fractionated on a sucrose gradient, a mRNA fraction of 2.5 kilobases expressed the Na+/P(i) cotransport activity in oocytes. This fraction resulted in a 6-fold stimulation of Na+-dependent P(i) transport when compared to oocytes injected with water. The K(m) and V(max) for Na+-dependent P(i) uptake were 0.18 mM and 118 pmol/oocyte per 30 min, respectively.
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页码：83 / 86
页数：4
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