HELP (HIGH-EFFICIENCY LIQUID-PHASE) NEW OLIGONUCLEOTIDE SYNTHESIS ON SOLUBLE POLYMERIC SUPPORT

被引：72

作者：

BONORA, GM ^{[1
]}

SCREMIN, CL ^{[1
]}

COLONNA, FP ^{[1
]}

GARBESI, A ^{[1
]}

机构：

[1] ICOCEA,CNR,I-40064 OZZANO EMILIA,ITALY

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 11期

关键词：

D O I：

10.1093/nar/18.11.3155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach Is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis withnew interesting advantages.Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalised PEG. © 1990 Oxford University Press.

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页码：3155 / 3159

页数：5

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